Description

  • Product name

    Recombinant Human CENPB protein
  • Biological activity

    ab73636 reacts with Scleroderma patient sera.
  • Purity

    > 90 % SDS-PAGE.
    ab73636 is purified by proprietary chromatographic techniques.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab73636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

    ab73636 reacts with Scleroderma patient sera.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    pH: 7.60
    Constituents: 0.476% HEPES, 20% Glycerol, 1.45% Sodium chloride

General Info

  • Alternative names

    • CENP B
    • CENP-B
    • CENPB
    • CENPB_HUMAN
    • centromere autoantigen B
    • Centromere protein B
    • Centromere protein B, 80kDa
    • Major centromere autoantigen B
    see all
  • Function

    Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
  • Sequence similarities

    Contains 1 HTH CENPB-type DNA-binding domain.
    Contains 1 HTH psq-type DNA-binding domain.
  • Cellular localization

    Nucleus. Chromosome > centromere.
  • Information by UniProt

References

This product has been referenced in:

  • Mohibi S  et al. Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation. J Biol Chem 290:28299-310 (2015). Read more (PubMed: 26429915) »
  • Favoino E  et al. Autoantibodies recognizing the amino terminal 1-17 segment of CENP-A display unique specificities in systemic sclerosis. PLoS One 8:e61453 (2013). WB . Read more (PubMed: 23613856) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73636.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up