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    recombinant-human-cerk-protein-ab196089.pdf

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Recombinant human CERK protein (ab196089)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human CERK protein (ab196089)
  • Functional Studies - Recombinant human CERK protein (ab196089)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 40% SDS-PAGE
  • Active: Yes
  • Tags: DDDDK tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CERK protein
    See all CERK proteins and peptides
  • Biological activity

    Specific Activity: 142 pmole/min/µg. 

    A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C. 

     

    CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected.

  • Purity

    >= 40 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q8TCT0
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADAC SVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWA QVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIY ERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMF SEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVG TSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDS EKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAG CFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCAC RRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWN CDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS
    • Predicted molecular weight

      60 kDa including tags
    • Amino acids

      1 to 537
    • Tags

      DDDDK tag C-Terminus
    • Additional sequence information

      NM_022766.

Associated products

  • Related Products

    • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
    • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)
    • Anti-CERK antibody (ab155061)
    • Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)

Specifications

Our Abpromise guarantee covers the use of ab196089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol, 0.05% DTT

    Contains 40 ug/ml FLAG peptide.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Acylsphingosine kinase
    • Ceramide kinase
    • Cerk
    • CERK1_HUMAN
    • dA59H18.2
    • dA59H18.3
    • hCERK
    • KIAA1646
    • Lipid kinase 4
    • Lipid kinase LK4
    • LK4
    see all
  • Function

    Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
  • Tissue specificity

    High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
  • Sequence similarities

    Contains 1 DAGKc domain.
  • Cellular localization

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession Q8TCT0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human CERK protein (ab196089)
    SDS-PAGE - Recombinant human CERK protein (ab196089)

    10% SDS-PAGE analysis of 5.6 µg ab196089 followed by Coomassie staining.

  • Functional Studies - Recombinant human CERK protein (ab196089)
    Functional Studies - Recombinant human CERK protein (ab196089)

    Kinase assay of ab196089 showing specific activity as 142 pmol/min/µg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab196089? Please let us know so that we can cite the reference in this datasheet.

    ab196089 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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