Recombinant human CERK protein (ab196089)
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Description
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Product name
Recombinant human CERK protein -
Biological activity
Specific Activity: 142 pmole/min/µg.
A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.
CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected.
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Purity
>= 40 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADAC SVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWA QVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIY ERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMF SEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVG TSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDS EKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAG CFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCAC RRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWN CDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS -
Predicted molecular weight
60 kDa including tags -
Amino acids
1 to 537 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
NM_022766.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol, 0.05% DTT
Contains 40 ug/ml FLAG peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Acylsphingosine kinase
- Ceramide kinase
- Cerk
see all -
Function
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. -
Tissue specificity
High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung. -
Sequence similarities
Contains 1 DAGKc domain. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human CERK protein (ab196089)10% SDS-PAGE analysis of 5.6 µg ab196089 followed by Coomassie staining.
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Functional Studies - Recombinant human CERK protein (ab196089)Kinase assay of ab196089 showing specific activity as 142 pmol/min/µg.
References
ab196089 has not yet been referenced specifically in any publications.
