Recombinant Human CERT protein (ab95897)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human CERT protein
See all CERT proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab95897 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF -
Predicted molecular weight
33 kDa including tags -
Amino acids
347 to 598 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab95897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF-TOF -
Form
Liquid -
Additional notes
Previously labelled as COL4A3BP.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- Alpha 3 type IV collagen binding protein
- C43BP_HUMAN
- Ceramide transfer protein
see all -
Function
May mediate the intracellular trafficking of ceramide in a non-vesicular manner. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 PH domain.
Contains 1 START domain. -
Domain
The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB. -
Post-translational
modificationsPhosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. -
Cellular localization
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab95897 has been referenced in 1 publication.
- Fleury L et al. Development of a CERT START Domain-Ceramide HTRF Binding Assay and Application to Pharmacological Studies and Screening. J Biomol Screen 20:779-87 (2015). PubMed: 25716975