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    recombinant-human-cert-protein-ab95897.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Recombinant Human CERT protein (ab95897)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (1)

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SDS-PAGE - Recombinant Human CERT protein (ab95897)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human CERT protein
      See all CERT proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      ab95897 is purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      Q9Y5P4
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF
      • Predicted molecular weight

        33 kDa including tags
      • Amino acids

        347 to 598
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab95897 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF-TOF
    • Form

      Liquid
    • Additional notes

      Previously labelled as COL4A3BP. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • Alpha 3 type IV collagen binding protein
      • C43BP_HUMAN
      • Ceramide transfer protein
      • Ceramide transporter
      • CERT
      • Cert protein
      • CERTL
      • col4a3bp
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein
      • Collagen type IV alpha 3 binding protein
      • Collagen type IV alpha-3-binding protein
      • FLJ20597
      • Goodpasture antigen binding protein
      • Goodpasture antigen-binding protein
      • GPBP
      • hCERT
      • Lipid transfer protein CERTL
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein
      • StAR related lipid transfer (START) domain containing 11
      • StAR related lipid transfer protein 11
      • StAR-related lipid transfer protein 11
      • StARD 11
      • StARD11
      • START domain containing 11
      • START domain containing protein 11
      • START domain-containing protein 11
      see all
    • Function

      May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 START domain.
    • Domain

      The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • Post-translational
      modifications

      Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • Cellular localization

      Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Target information above from: UniProt accession Q9Y5P4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CERT protein (ab95897)
      SDS-PAGE - Recombinant Human CERT protein (ab95897)
      15% SDS-PAGE showing ab95897 at approximately 33.1kDa (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab95897? Please let us know so that we can cite the reference in this datasheet.

    ab95897 has been referenced in 1 publication.

    • Fleury L  et al. Development of a CERT START Domain-Ceramide HTRF Binding Assay and Application to Pharmacological Studies and Screening. J Biomol Screen 20:779-87 (2015). PubMed: 25716975

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Question

    Je souhaiterais savoir si ce type de protéine présente une certaine instabilité quant à l'utilisation de tampon PBS ou à l'élimination du glycerol.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2013

    Answer

    Nous n'avons pas essayé cette protéine dans un tampon PBS donc nous ne savons pas si cette protéine devient instable dans ce tampon. Cependant, le laboratoire nous a dit qu’il est recommandé de garder le glycerol dans le tampon pour stabiliser la protéine.

    Read More

    Abcam Scientific Support

    Answered on Jan 24 2013

    Question

    Nous vous avons commandé plusieurs lots de "COL4A3BP protein (His Tag)" et je souhaiterais connaître le coefficient d'absorption molaire à 280 nm de cette protéine. Pouvez-vous me le communiquer SVP?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2012

    Answer

    Merci de nous avoir contactés.

    Nous n'avons pas mesuré le coefficient d'absorption molaire exact à 280 nm, mais voici quelques informations à propos de la valeur théorique.

    Selon l'outil ProtParam Expasy (http://web.expasy.org/protparam/):

    Les coefficients d'extinction:

    (Les coefficients d'extinction sont en M-1 cm-1, à 280 nm mesurés dans de l'eau.)

    Ext. coefficient 57660

    Abs 0,1% (= 1 g / l) 1,750, en supposant que toutes les paires de résidus Cys forment des ponts disulfures

    Couleur ext. coefficient 57410

    Abs 0,1% (= 1 g / l) 1,743, en supposant que tous les résidus Cys sont réduits

    Selon http://www.pveducation.org/pvcdrom/pn-junction/absorption-coefficient le coefficient d'absorption molaire est calculé comme suit:

    Le coefficient d'absorption, α, est en relation avec le coefficient d'extinction, k, par la formule suivante:

    α = 4πk/λ

    où λ est la longueur d'onde. Si λ est en nm, il faut multiplier par 107 pour obtenir le coefficient d'absorption dans les unités de cm-1.

    Cela devrait correspondre à un coefficient d'absorption molaire théorique de 4117,9 M-1 cm-1 à 280 nm.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 13 2012

    Question

    Nous vous avons commandé plusieurs lots de "COL4A3BP protein (His Tag)" et je souhaiterais connaître le coefficient d'absorption molaire à 280 nm de cette protéine. Pouvez-vous me le communiquer SVP?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 11 2012

    Answer

    Merci de nous avoir contactés.

    Nous avons deux protéines au catalogue avec ce nom : ab40265 et ab95897. Pourriez-vous me dire pour laquelle de ces références vous souhaitez connaître le coefficient d'absorption molaire à 280 nm ?

    Read More

    Abcam Scientific Support

    Answered on Dec 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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