Description

  • Product name

    Recombinant Human CHFR protein
  • Expression system

    Wheat germ
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      TSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSP AGRERSSSC
    • Amino acids

      136 to 194
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab163200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • Checkpoint with forkhead and ring finger domains
    • Checkpoint with forkhead and RING finger domains protein
    • Chfr
    • CHFR_HUMAN
    • E3 ubiquitin protein ligase CHFR
    • E3 ubiquitin-protein ligase CHFR
    • EC 6.3.2.
    • FLJ10796
    • FLJ33629
    • RING finger protein 196
    • RNF 116
    • RNF 196
    • RNF116
    • RNF196
    • Ubiquitin ligase protein
    see all
  • Function

    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
  • Tissue specificity

    Ubiquitous.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the CHFR family.
    Contains 1 FHA domain.
    Contains 1 PBZ-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Developmental stage

    Weakly expressed in G1 phase, and highly expressed during S phase.
  • Domain

    The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
    The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.
  • Post-translational
    modifications

    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Autoubiquitinated; may regulate its cellular level.
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
  • Cellular localization

    Nucleus > PML body.
  • Information by UniProt

Images

  • ab163200 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab163200 has not yet been referenced specifically in any publications.

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