Description

  • Product name

    Recombinant Human CHMP1a protein
  • Purity

    > 85 % SDS-PAGE.
    ab139206 was purified by using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN
    • Predicted molecular weight

      24 kDa including tags
    • Amino acids

      1 to 196
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab139206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • Charged multivesicular body protein 1a
    • CHM1A_HUMAN
    • CHMP1a
    • Chromatin modifying protein 1a
    • Chromatin-modifying protein 1a
    • hVps46 1
    • hVps46-1
    • KIAA0047
    • Metalloprotease 1
    • PCOLN3
    • Procollagen (type III) N endopeptidase
    • PRSM1
    • Vacuolar protein sorting 46 1
    • Vacuolar protein sorting-associated protein 46-1
    • Vps46 1
    • Vps46-1
    see all
  • Function

    Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing.
  • Tissue specificity

    Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63.
  • Sequence similarities

    Belongs to the SNF7 family.
  • Cellular localization

    Cytoplasm. Endosome membrane. Nucleus matrix. The cytoplasmic form is partially membrane-associated and localizes to early endosomes. The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab139206 (3µg)

References

ab139206 has not yet been referenced specifically in any publications.

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