Description

  • Product name

    Recombinant Human Chromogranin A protein
    See all Chromogranin A proteins and peptides
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLP VNSPMNKGDT EVMKCIVEVI SDTLSKPSPM PVSQECFETL RGDERILSIL RHQNLLKELQ DLALQGAKER AHQQKKHSGF EDELSEVLEN QSSQAELKEA VEEPSSKDVM EKREDSKEAE KSGEATDGAR PQALPEPMQE SKAEGNNQAP GEEEEEEEEA TNTHPPASLP SQKYPGPQAE GDSEGLSQGL VDREKGLSAE PGWQAKREEE EEEEEEAEAG EEAVPEEEGP TVVLNPHPSL GYKEIRKGES RSEALAVDGA GKPGAEEAQD PEGKGEQEHS QQKEEEEEMA VVPQGLFRGG KSGELEQEEE RLSKEWEDSK RWSKMDQLAK ELTAEKRLEG QEEEEDNRDS SMKLSFRARA YGFRGPGPQL RRGWRPSSRE DSLEAGLPLQ VRGYPEEKKE EEGSANRRPE DQELESLSAI EAELEKVAHQ LQALRRG
    • Amino acids

      19 to 457
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab85486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 6.00
    Constituents: 0.39% MES, 0.00174% PMSF, 0.0584% EDTA, 10% Glycerol

General Info

  • Alternative names

    • beta Granin
    • betagranin (N-terminal fragment of chromogranin A)
    • catestatin
    • CgA
    • CHG A
    • Chga
    • chromofungin
    • Chromogranin A
    • Chromogranin A parathyroid secretory protein 1
    • Chromogranin A precursor
    • ChromograninA
    • CMGA_HUMAN
    • ER-37
    • Pancreastatin
    • Parastatin
    • Parathyroid secretory protein 1
    • Pituitary secretory protein I
    • Secretory protein I
    • SP I
    • SP-I
    • SP1
    • SPI
    • Vasostatin
    • vasostatin 2
    • Vasostatin I
    • Vasostatin II
    • vasostatin-2
    see all
  • Function

    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications

    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt

Images

  • Standard curve for Chromogranin A (Analyte: ab85486); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab36997 at 1µg/ml and Detector Antibody ab17064 at 0.5µg/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline.
  • 12% SDS-PAGE analysis of 3µg of ab85486
  • Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.

References

This product has been referenced in:

  • Noborn F  et al. Identification of chondroitin sulfate linkage region glycopeptides reveals prohormones as a novel class of proteoglycans. Mol Cell Proteomics 14:41-9 (2015). Read more (PubMed: 25326458) »
See 1 Publication for this product

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