Recombinant Human CHST15 protein (ab162523)
Key features and details
- Expression system: Wheat germ
- Purity: > 80% Purified via GST Tag
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
Description
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Product name
Recombinant Human CHST15 protein
See all CHST15 proteins and peptides -
Purity
> 80 % Purified via GST Tag.
Purification: Glutathione Sepharose 4 Fast Flow; the purity will be >80% (by CBR). -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRHCINCCIQLLPDGAHKQQVNCQGGPHHGHQACPTCKGENKILFRVDSK QMNLLAVLEVRTEGNENWGGFLRFKKGKRCSLVFGLIIMTLVMASYILSG AHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYP SIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCW YEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRL RCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRL RDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASAS TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYS DYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPV RLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGP LSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQV LADEAFAWKTT -
Amino acids
1 to 561 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab162523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- B cell RAG associated gene protein
- B cell RAG associated protein
- B-cell RAG-associated gene protein
see all -
Function
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. -
Tissue specificity
Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. -
Sequence similarities
Belongs to the sulfotransferase 1 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab162523 has not yet been referenced specifically in any publications.