Recombinant Human CHST15 protein (ab162523)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human CHST15 protein -
Purity
> 80 % Purified via GST Tag.
Purification: Glutathione Sepharose 4 Fast Flow; the purity will be >80% (by CBR). -
Expression system
Wheat germ -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRHCINCCIQLLPDGAHKQQVNCQGGPHHGHQACPTCKGENKILFRVDSK QMNLLAVLEVRTEGNENWGGFLRFKKGKRCSLVFGLIIMTLVMASYILSG AHQELLISSPFHYGGFPSNPSLMDSENPSDTKEHHHQSSVNNISYMKDYP SIKLIINSITTRIEFTTRQLPDLEDLKKQELHMFSVIPNKFLPNSKSPCW YEEFSGQNTTDPYLTNSYVLYSKRFRSTFDALRKAFWGHLAHAHGKHFRL RCLPHFYIIGQPKCGTTDLYDRLRLHPEVKFSAIKEPHWWTRKRFGIVRL RDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASAS TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVERLYS DYLYFASSNKSADDFHEKVTEALQLFENCMLDYSLRACVYNNTLNNAMPV RLQVGLYAVYLLDWLSVFDKQQFLILRLEDHASNVKYTMHKVFQFLNLGP LSEKQEALMTKSPASNARRPEDRNLGPMWPITQKILRDFYRPFNARLAQV LADEAFAWKTT -
Amino acids
1 to 561 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab162523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- B cell RAG associated gene protein
- B cell RAG associated protein
- B-cell RAG-associated gene protein
see all -
Function
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. -
Tissue specificity
Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. -
Sequence similarities
Belongs to the sulfotransferase 1 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CHST15 protein (ab162523)ab162523 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Datasheets and documents
References
ab162523 has not yet been referenced specifically in any publications.
