Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameRecombinant Human CI009 protein (Tagged)
Purity> 90 % SDS-PAGE.
ab199579 was refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVK DALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSE EGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIAR RHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSG GSHHHHHHGSENLYFQGEFNEVKESLRSIEQKYKLFQQQQLTFTAALEHC RENAHDKIRPISSIGQVQSYMEHYCNSSTDRRVLLMFLDICSELNKLCQH FEAVHSGTPVTNNLLEKCKTLVSQSNDLSSLRAKYPHDVVNHLSCDEARN HYGGVVSLIPLILDLMKEWIAHSEKLPRKVLQHVSEPQAHQESTRGAARP AQAIGTQPRATKHKCRQLTKASLKPRGCSKPPWRPPGGKL
Predicted molecular weight50 kDa including tags
Amino acids2 to 165
Additional sequence informationFull length protein without starter Methionine constucted with Human Alpha-Fetal Protein N-terminal domain (AFPn)-His-TEV cleavage site (219amino acids) fusion at its N-terminal.
Our Abpromise guarantee covers the use of ab199579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, Sucrose and DTT.
- Chromosome 9 open reading frame 9
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab199579 has not yet been referenced specifically in any publications.