Recombinant Human CISH/CIS protein (ab117057)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human CISH/CIS protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGT PAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMP EGTFLVRDSTHPSYLFTLSVKTTRGPTNVCIEYADSSFRLDSNCLSRPRI LAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAP PPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMAGY LRQYPFQL -
Predicted molecular weight
54 kDa including tags -
Amino acids
1 to 258
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab117057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
This protein is best used within three months from the date of receipt.
This product was previously labelled as CISH.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- BACTS2
- CIS
- cis 1
see all -
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Post-translational
modificationsAssociation with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa). - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CISH/CIS protein (ab117057)12.5% SDS-PAGE showing ab117057 at approximately 54.49 kDa.
Stained with Coomassie Blue.
Datasheets and documents
References
ab117057 has not yet been referenced specifically in any publications.
