Recombinant human CLK3 protein (ab85759)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CLK3 protein -
Biological activity
The Specific activity of ab85759 was determined to be 75 nmol/min/mg.
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Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab85759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CDC like kinase 3
- CDC-like kinase 3
- CLK3
see all -
Function
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylates on all three types of residues. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. - Information by UniProt
Images
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The specific activity of CLK3 (ab85759) was determined to be 28.9 nmol/min/mg as per activity assay protocol and was equivalent to 80 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab85759
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The Specific activity of ab85759 was determined to be 75 nmol/min/mg.
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SDS-PAGE showing ab85759 at approximately 86kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85759 has not yet been referenced specifically in any publications.