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Recombinant Human Clusterin protein (ab177715)

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  • SDS
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SDS-PAGE - Recombinant Human Clusterin   protein (ab177715)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human Clusterin protein
      See all Clusterin proteins and peptides
    • Purity

      > 85 % SDS-PAGE.
      ab177715 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P10909
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSDQTV SDNELQEMSN QGSKYVNKEI QNAVNGVKQI KTLIEKTNEE RKTLLSNLEE AKKKKEDALN ETRESETKLK ELPGVCNETM MALWEECKPC LKQTCMKFYA RVCRSGSGLV GRQLEEFLNQ SSPFYFWMNG DRIDSLLEND RQQTHMLDVM QDHFSRASSI IDELFQDRFF TREPQDTYHY LPFSLPHRRP HFFFPKSRIV RSLMPFSPYE PLNFHAMFQP FLEMIHEAQQ AMDIHFHSPA FQHPPTEFIR EGDDDRTVCR EIRHNSTGCL RMKDQCDKCR EILSVDCSTN NPSQAKLRRE LDESLQVAER LTRKYNELLK SYQWKMLNTS SLLEQLNEQF NWVSRLANLT QGEDQYYLRV TTVASHTSDS DVPSGVTEVV VKLFDSDPIT VTVPVEVSRK NPKFMETVAE KALQEYRKKH REE
      • Predicted molecular weight

        54 kDa including tags
      • Amino acids

        23 to 449
      • Tags

        His tag N-Terminus
      • Additional sequence information

        Mature form. NP_001822.

    Associated products

    • Related Products

      • Anti-Clusterin antibody (ab104652)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-Clusterin antibody (ab39991)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab177715 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.3152% Tris HCl, 0.88% Sodium chloride, 10% Glycerol, 0.02% DTT

    General Info

    • Alternative names

      • 40
      • AAG 4
      • AAG4
      • Aging associated protein 4
      • Aging-associated gene 4 protein
      • AI893575
      • APO J
      • Apo-J
      • APOJ
      • ApoJalpha
      • ApoJbeta
      • Apolipoprotein J
      • ApolipoproteinJ
      • CLI
      • CLU
      • CLU1
      • CLU2
      • CLUS_HUMAN
      • Clusterin
      • Clusterin alpha chain
      • Clusterin beta chain
      • Complement associated protein SP 40
      • Complement associated protein SP 40 40
      • Complement associated protein SP40
      • Complement cytolysis inhibitor
      • Complement cytolysis inhibitor a chain
      • Complement cytolysis inhibitor b chain
      • Complement lysis inhibitor
      • Complement-associated protein SP-40
      • D14Ucla3
      • Dimeric acid glycoprotein
      • Glycoprotein 80
      • Glycoprotein III
      • GP80
      • Ku70-binding protein 1
      • KUB 1
      • KUB1
      • MGC24903
      • NA1/NA2
      • RATTRPM2B
      • SGP 2
      • SGP2
      • SP 40
      • SP40
      • Sugp-2
      • Sulfated glycoprotein 2
      • Testosterone repressed prostate message 2
      • Testosterone-repressed prostate message 2
      • TRPM 2
      • TRPM-2
      • TRPM2
      • TRPM2B
      • Trpmb
      see all
    • Function

      Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
    • Tissue specificity

      Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
    • Sequence similarities

      Belongs to the clusterin family.
    • Post-translational
      modifications

      Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
      Polyubiquitinated, leading to proteasomal degradation.
      Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
    • Cellular localization

      Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis.
    • Target information above from: UniProt accession P10909 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Clusterin   protein (ab177715)
      SDS-PAGE - Recombinant Human Clusterin protein (ab177715)

      15% SDS-PAGE analysis of ab177715 (3μg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab177715? Please let us know so that we can cite the reference in this datasheet.

    ab177715 has not yet been referenced specifically in any publications.

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