Description

  • Product name

    Recombinant human CNTF protein (Active) (Animal Free)
    See all CNTF proteins and peptides
  • Biological activity

    The activity can be determined by the dose-dependent stimulation of TF-1 cells and is typically less than 25 ng/mL (corresponding to the specific activity of 4 x 105 units/mg). 

  • Purity

    > 95 % SDS-PAGE.
    The purity of ab218081 is typically 98% as determined by HPLC, reducing and non-reducing SDS-PAGE, UV spectroscopy at 280 nm.
  • Endotoxin level

    < 0.050 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
    • Predicted molecular weight

      23 kDa
    • Amino acids

      1 to 200
  • Description

    Recombinant human CNTF protein (Active)

Specifications

Our Abpromise guarantee covers the use of ab218081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term.

    pH: 7.5
    Constituent: 0.14% Sodium phosphate

    Lyophilized from a sterile filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Sterile water at 100µg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting sterile distilled water down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.

General Info

  • Alternative names

    • Ciliary neurotrophic factor
    • CNTF
    • CNTF_HUMAN
    • HCNTF
  • Function

    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
  • Tissue specificity

    Nervous system.
  • Sequence similarities

    Belongs to the CNTF family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • Functional analysis of ab218081
  •  SDS PAGE analysis of ab218081 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab218081 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218081.
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