Recombinant Human COASY protein (ab211940)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human COASY protein -
Purity
> 90 % SDS-PAGE.
This protein was expressed in E. coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGEFMAINRFRLENDLEELALYQIQLLKDLRHTENEEDK VSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKG LGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRV FGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQ NLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHV VLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD -
Predicted molecular weight
32 kDa including tags -
Amino acids
296 to 564 -
Tags
T7 tag N-Terminus -
Additional sequence information
Constructed with 15 N-terminal T7 tag.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab211940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, Sucrose and DTT.
General Info
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Alternative names
- Bifunctional coenzyme A synthase
- Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase
- CoA synthase
see all -
Function
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. -
Tissue specificity
Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain. -
Pathway
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5. -
Sequence similarities
In the central section; belongs to the eukaryotic coaD family.
Contains 1 DPCK (dephospho-CoA kinase) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab211940 has not yet been referenced specifically in any publications.
