Key features and details
- Expression system: HEK 293 cells
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human COMP/Cartilage oligomeric matrix protein
See all COMP/Cartilage oligomeric matrix protein proteins and peptides
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceHVDYKDDDDKPAGQGQSPLGSDLGPQMLRELQETNAALQDVRE LLRQQ VREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGV ACI QTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFR C EACPPGYSGPTHQGVGLAFAKANKQVCTDINECETGQHNCVPNSVCINT RGSFQCGPCQPGFVGDQASGCQRRAQRFCPDGSPSECHEHADCVLERDG S RSCVCAVGWAGNGILCGRDTDLDGFPDEKLRCPERQCRKDNCVTVPN SGQ EDVDRDGIGDACDPDADGDGVPNEKDNCPLVRNPDQRNTDEDKWG DACDN CRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNQADNCPRVPNS DQKDSDG DGIGDACDNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGH QDSRDNCPT VPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPN PGQEDADRDGV GDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTV VLDPEGDAQIDPN WVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGT FHVNTVTDDDYAGFI FGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEP GIQLKAVKSSTGPGEQL RNALWHTGDTESQVRLLWKDPRNVGWKDKKS YRWFLQHRPQVGYIRVRFY EGPELVADSNVVLDTTMRGGRLGVFCFSQ ENIIWANLRYRCNDTIPEDYE THQLRQA
Predicted molecular weight82 kDa including tags
Amino acids21 to 757
TagsDDDDK tag N-Terminus
- Anti-COMP/Cartilage oligomeric matrix protein antibody [MA37C94 (HC484D1)] (ab11056)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-COMP/Cartilage oligomeric matrix protein antibody (ab42225)
- Anti-COMP/Cartilage oligomeric matrix protein antibody (ab74524)
Our Abpromise guarantee covers the use of ab104358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
This product was previously labelled as COMP
Concentration information loading...
Preparation and Storage
Stability and Storage
Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 0.242% Tris, 0.29% Sodium chloride
ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
- Cartilage oligomeric matrix protein
- cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
- Cartilage oligomeric matrix protein precursor
FunctionMay play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
Tissue specificityAbundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
Involvement in diseaseDefects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 8 TSP type-3 repeats.
Developmental stagePresent during the earliest stages of limb maturation and is later found in regions where the joints develop.
DomainThe cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
The TSP C-terminal domain mediates interaction with FN1 and ACAN.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
14% SDS-PAGE showing ab104358 at approximately 82.4kDa:
Lane 1: M.W. marker – 21, 31, 45, 66, 97 kDa.
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane.
Anti-COMP/Cartilage oligomeric matrix protein antibody (ab42225) at 1/1000 dilution +
Recombinant Human COMP/Cartilage oligomeric matrix protein (ab104358) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab104358 has been referenced in 2 publications.
- Crevenna AH et al. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. J Cell Biol 213:305-14 (2016). PubMed: 27138253
- Pu X et al. ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant. Am J Hum Genet 92:366-74 (2013). PubMed: 23415669