Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant Human Contactin 6 protein
Biological activityInduces Hes-1 in 3T3L-1 cells.
Purity> 90 % SDS-PAGE.
ab109345 is an 0.2 µm filtered solution.
Endotoxin level< 0.100 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
Predicted molecular weight150 kDa including tags
Amino acids1 to 999
Our Abpromise guarantee covers the use of ab109345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesInduces Hes-1 in 3T3L-1 cells.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- CNTN 6
FunctionContactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
Tissue specificityExpressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
Induction of Hes-1 with the treatment of hContactin-6-Fc.
A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 μg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).
Lane M: Marker
Lane 1: ab109345, 0 minutes
Lane 2: ab109345, 10 minutes
Lane 3: ab109345, 30 minutes
Lane 4: ab109345, 1 h
Lane 5: ab109345, 2 h
Lane 6: ab109345, 4 h
Lane 7: ab109345, 8 h
Lane 8: ab109345, 24 h
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab109345 has not yet been referenced specifically in any publications.