Recombinant Human COPE protein (ab187476)

Submit a review Submit a question

Description

  • Product name

    Recombinant Human COPE protein

  • Purity

    > 90 % SDS-PAGE.
    ab187476 was purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    O14579

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMGSMAPPAPGPASGGSGEVDELFDVKNAFY IGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSS APELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIY LHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDE DATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHM AQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQL KDAHRSHPFIKEYQAKENDFDRLVLQYAPSA

    • Predicted molecular weight

      37 kDa including tags

    • Amino acids

      1 to 308

    • Tags

      His tag N-Terminus

    • Additional sequence information

      NP_009194

Specifications

Our Abpromise guarantee covers the use of ab187476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 79% PBS, 20% Glycerol, 0.02% DTT

General Info

  • Alternative names

    • 1110005D17Rik
    • Coatomer epsilon subunit
    • Coatomer protein complex subunit epsilon
    • Coatomer subunit epsilon
    • COPE
    • COPE_HUMAN
    • Cope1
    • Epsilon coat protein
    • Epsilon COP
    • Epsilon COP I
    • Epsilon subunit of coatomer protein complex
    • Epsilon-coat protein
    • Epsilon-COP
    • FLJ13241
    see all

  • Function

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

  • Sequence similarities

    Belongs to the COPE family.

  • Post-translational
    modifications

    Phosphorylated by PKA.
    Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.

  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human COPE protein (ab187476)
    SDS-PAGE - Recombinant Human COPE protein (ab187476)

    15% SDS-PAGE analysis of 3 µg ab187476.

References

ab187476 has not yet been referenced specifically in any publications.

Publishing research using ab187476? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab187476.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com