Recombinant Human COPE protein (ab187476)

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SDS-PAGE - Recombinant Human COPE protein (ab187476)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human COPE protein

    • Purity

      > 90 % SDS-PAGE.
      ab187476 was purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      O14579

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMAPPAPGPASGGSGEVDELFDVKNAFY IGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSS APELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIY LHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDE DATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHM AQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQL KDAHRSHPFIKEYQAKENDFDRLVLQYAPSA

      • Predicted molecular weight

        37 kDa including tags

      • Amino acids

        1 to 308

      • Tags

        His tag N-Terminus

      • Additional sequence information

        NP_009194

    Specifications

    Our Abpromise guarantee covers the use of ab187476 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% DTT

    General Info

    • Alternative names

      • 1110005D17Rik
      • Coatomer epsilon subunit
      • Coatomer protein complex subunit epsilon
      • Coatomer subunit epsilon
      • COPE
      • COPE_HUMAN
      • Cope1
      • Epsilon coat protein
      • Epsilon COP
      • Epsilon COP I
      • Epsilon subunit of coatomer protein complex
      • Epsilon-coat protein
      • Epsilon-COP
      • FLJ13241
      see all

    • Function

      The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

    • Sequence similarities

      Belongs to the COPE family.

    • Post-translational
      modifications

      Phosphorylated by PKA.
      Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.

    • Cellular localization

      Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human COPE protein (ab187476)
      SDS-PAGE - Recombinant Human COPE protein (ab187476)

      15% SDS-PAGE analysis of 3 µg ab187476.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab187476? Please let us know so that we can cite the reference in this datasheet.

    ab187476 has not yet been referenced specifically in any publications.

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