Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Tags: His tag C-Terminus
Description
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Product name
Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) -
Purity
>= 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 419 -
Tags
His tag C-Terminus -
Additional sequence information
N-terminal MSKIKGS, C-terminal EFGGGS 8xHis
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab273530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Form
Lyophilized -
Additional notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at -80°C.
Information available upon request.
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ReconstitutionReconstitute in Phosphate Buffered saline, aliquot and store at -80 C for 12 months or +4 C for 1 week. Avoid repeated freeze thaw.
General Info
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Alternative names
- 2019-nCoV nucleocapsid protein
- N
- NC
see all -
Relevance
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
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Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab273530 has not yet been referenced specifically in any publications.