Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)

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Description

  • Product name

    Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

  • Purity

    >= 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P0DTC9

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human coronavirus

    • Sequence

      MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA

    • Predicted molecular weight

      48 kDa including tags

    • Amino acids

      1 to 419

    • Tags

      His tag C-Terminus

    • Additional sequence information

      N-terminal MSKIKGS, C-terminal EFGGGS 8xHis

Specifications

Our Abpromise guarantee covers the use of ab273530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Lyophilized

  • Additional notes

    This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -80°C.

    Constituents: Trehalose, 0.99% Potassium phosphate

    Buffer lyophilized from.

  • Reconstitution
    Reconstitute in Phosphate Buffered saline, aliquot and store at -80 C for 12 months or +4 C for 1 week. Avoid repeated freeze thaw.

General Info

  • Alternative names

    • 2019-nCoV nucleocapsid protein
    • NC
    • Nucleoprotein
    • Protein N

  • Relevance

    Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.

Images

  • SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
    SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)

    SDS-PAGE analysis of ab273530.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab273530? Please let us know so that we can cite the reference in this datasheet.

ab273530 has not yet been referenced specifically in any publications.

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