Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human coronavirus SARS-CoV-2 RNA-dependent RNA polymerase (RDRP) protein (Active)
See all RNA-dependent RNA polymerase (RDRP) proteins and peptides
The specific activity of ab277617 was determined by detection of pyrophosphate (PPi) generated during nascent strand synthesis by a chemiluminescent coupled assy method using ATP sulfurylase and luciferase.
Purity> 85 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceSADAQSFLNR VCGVSAARLT PCGTGTSTDV VYRAFDIYND KVAGFAKFLK TNCCRFQEKD EDDNLIDSYF VVKRHTFSNY QHEETIYNLL KDCPAVAKHD FFKFRIDGDM VPHISRQRLT KYTMADLVYA LRHFDEGNCD TLKEILVTYN CCDDDYFNKK DWYDFVENPD ILRVYANLGE RVRQALLKTV QFCDAMRNAG IVGVLTLDNQ DLNGNWYDFG DFIQTTPGSG VPVVDSYYSL LMPILTLTRA LTAESHVDTD LTKPYIKWDL LKYDFTEERL KLFDRYFKYW DQTYHPNCVN CLDDRCILHC ANFNVLFSTV FPPTSFGPLV RKIFVDGVPF VVSTGYHFRE LGVVHNQDVN LHSSRLSFKE LLVYAADPAM HAASGNLLLD KRTTCFSVAA LTNNVAFQTV KPGNFNKDFY DFAVSKGFFK EGSSVELKHF FFAQDGNAAI SDYDYYRYNL PTMCDIRQLL FVVEVVDKYF DCYDGGCINA NQVIVNNLDK SAGFPFNKWG KARLYYDSMS YEDQDALFAY TKRNVIPTIT QMNLKYAISA KNRARTVAGV SICSTMTNRQ FHQKLLKSIA ATRGATVVIG TSKFYGGWHN MLKTVYSDVE NPHLMGWDYP KCDRAMPNML RIMASLVLAR KHTTCCSLSH RFYRLANECA QVLSEMVMCG GSLYVKPGGT SSGDATTAYA NSVFNICQAV TANVNALLST DGNKIADKYV RNLQHRLYEC LYRNRDVDTD FVNEFYAYLR KHFSMMILSD DAVVCFNSTY ASQGLVASIK NFKSVLYYQN NVFMSEAKCW TETDLTKGPH EFCSQHTMLV KQGDDYVYLP YPDPSRILGA GCFVDDIVKT DGTLMIERFV SLAIDAYPLT KHPNQEYADV FHLYLQYIRK LHDELTGHML DMYSVMLTND NTSRYWEPEF YEAMYTPHTV LQ
Molecular weight information100 kDa by SDS-PAGE
Amino acids4393 to 5324
TagsHis tag C-Terminus
Additional sequence informationThe gene accession number is QHD43415.
DescriptionRecombinant human coronavirus RNA-dependent RNA polymerase (RDRP) protein (Active)
Our Abpromise guarantee covers the use of ab277617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.02% Imidazole
Constituents: 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.0015% DTT, 86.485% Water
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Protein rdp 1
- Protein rdp1
- Rdp 1
RelevanceRdRP plays a role in the RNA interference (RNAi) pathway. RNAi is the process of sequence-specific,post-transcriptional gene silencing initiated by double-stranded RNA (dsRNA). Recent studies showed small interfering RNAs (siRNA) generated by Dicer from longer dsRNA specifically suppressed expression of genes in somatic and embryo cells. An RdRP activity might provide amplification by replication of long trigger dsRNAs or copying of short siRNAs in a primer-independent manner. The siRNA primed RdRP reaction converts target mRNA into dsRNA, as well as possibly replicating trigger dsRNA. Both products then serve as DICER substrates, initiating the RdRP chain reaction. RNAi is important for heterochromatin formation and accurate chromosome segregation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277617 has not yet been referenced specifically in any publications.