Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (Active) (ab289629)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (Active)
See all SARS-CoV-2 Spike Glycoprotein S1 proteins and peptides -
Biological activity
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 µg/mL (100 µL/well) can bind ab289629, the EC50 of ab289629 is 100-600 ng/mL.
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Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Predicted molecular weight
77 kDa -
Molecular weight information
ab289629 consists of 681 amino acids and predicts a molecular mass of 76.5 kDa. As a result of glycosylation, it migrates as an approximately 112.2 kDa band in SDS-PAGE under reducing conditions. -
Amino acids
1 to 685 -
Modifications
mutated Q677H -
Tags
His tag C-Terminus -
Additional sequence information
YP_009724390.1
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Specifications
Our Abpromise guarantee covers the use of ab289629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 89% PBS, 5% Mannitol, 5% Trehalose, 0.01% Tween 80
The formulation is lot specific. Please contact our Technical Support team for details.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionLot specific
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab289629 has not yet been referenced specifically in any publications.