Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270825)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: GST tag C-Terminus
- Suitable for: ELISA, WB, SDS-PAGE
Description
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Product name
Recombinant Human coronavirus SARS Nucleocapsid Protein
See all SARS Nucleocapsid Protein proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Predicted molecular weight
32 kDa -
Amino acids
1 to 49 -
Tags
GST tag C-Terminus -
Additional sequence information
SARS Coronavirus Nucleoprotein (N-Term). Contains Nucleocapsid core protein, 1-49 amino acids immunodominant regions.
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Specifications
Our Abpromise guarantee covers the use of ab270825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituents: 0.35% Sodium chloride, 50% Glycerol (glycerin, glycerine), 0.79% Tris HCl
General Info
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Alternative names
- N
- NC
- Nucleocapsid protein
see all -
Relevance
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA. -
Cellular localization
Inside the virion, complexed with the viral RNA. May be associated with cellular membranes where it participates in viral RNA synthesis and virus budding.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab270825 has not yet been referenced specifically in any publications.