Recombinant Human Cortisol Binding Globulin protein (ab153766)

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Overview

Description

  • Nature

    Recombinant

  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
      P08185

    • Species

      Human

    • Sequence

      MDPNAAYVNMSNHHRGLASANVDFAFSLYKHLVALSPKKNIFISPVSISM ALAMLSLGTCGHTRAQLLQGLGFNLTERSETEIHQGFQHLHQLFAKSDTS LEMTMGNALFLDGSLELLESFSADIKHYYESEVLAMNFQDWATASRQINS YVKNKTQGKIVDLFSGLDSPAILVLVNYIFFKGTWTQPFDLASTREENFY VDETTVVKVPMMLQSSTISYLHDAELPCQLVQMNYVGNGTVFFILPDKGK MNTVIAALSRDTINRWSAGLTSSQVDLYIPKVTISGVYDLGDVLEEMGIA DLFTNQANFSRITQDAQLKSSKVVHKAVLQLNEEGVDTAGSTGVTLNLTS KPIILRFNQPFIIMIFDHFTWSSLFLARVMNPVLDHHHHHH

    • Molecular weight

      44 kDa including tags

    • Amino acids

      23 to 405

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    HPLC

  • Endotoxin level

    < 1.000 Eu/µg

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab153766 was lyophilised from a 0.2 µM filtered solution.

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilised protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at < -20°C.

General Info

  • Alternative names

    • CBG
    • CBG_HUMAN
    • corticosteroid binding globulin
    • Corticosteroid-binding globulin
    • serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6
    • Serpin A6
    • serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6
    • Serpina6
    • Transcortin
    see all

  • Function

    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.

  • Tissue specificity

    Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.

  • Involvement in disease

    Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.

  • Sequence similarities

    Belongs to the serpin family.

  • Post-translational
    modifications

    N-glycosylated; binds 5 oligosaccharide chains.
    Glycosylation in position Asn-260 is needed for steroid binding.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab153766 has not yet been referenced specifically in any publications.

Publishing research using ab153766? Please let us know so that we can cite the reference in this datasheet.

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