Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human COX1 / Cyclooxygenase 1 protein
Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 µM EDTA) with 5 µM Hematin for 10 min
then added 100 µM Ampliflu Red and 200 μM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5
minutes at room temperature. Reaction volume was 100 μl. Application: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMSRSLLLWFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFG LDRYQCDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWE FVNATFIREMLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRI LPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFAFF AQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGK LKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLY ATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQ LSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQE YSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDV IRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDAL EFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFG GEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTELD YKDDDDKHHHHHH
Predicted molecular weight71 kDa including tags
Amino acids1 to 599
TagsHis-DDDDK tag C-Terminus
Additional sequence informationNM_000962.
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.08% Triton X-100, 0.0067% Diethyldithiocarbamate
Contains 80 µg/ml DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- COX 1
- COX 3
FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
PathwayLipid metabolism; prostaglandin biosynthesis.
Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
4-20% SDS-PAGE analysis of 1.2 µg ab198643 with Coomassie staining.
Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 µM EDTA) with 5 µM Hematin for 10 min then added 100 µM Ampliflu Red and 200 μM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 μl.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198643 has not yet been referenced specifically in any publications.