Description

  • Product name

    Recombinant human Creatine kinase B type protein
    See all Creatine kinase B type proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab73647 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • Expression system

    Pichia pastoris
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab73647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 6.20
    Constituents: 0.0077% DTT, 0.158% Tris HCl, 0.0146% EDTA, 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • B CK
    • B-CK
    • BB-CK
    • BCK
    • Brain creatine kinase
    • Ckb
    • CKBB
    • Creatine kinase B
    • Creatine kinase B chain
    • Creatine kinase B type
    • Creatine kinase B-type
    • Creatine Kinase BB Isoenzyme
    • Creatine kinase brain
    • Creatine kinase brain type
    • Creatine phosphokinase BB
    • Epididymis luminal protein 211
    • Epididymis secretory protein Li 29
    • HEL 211
    • HEL S 29
    • KCRB_HUMAN
    see all
  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

References

ab73647 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your call earlier this week and for your patience while I have been in touch with the lab that produces this protein. My contact at the production lab did not have any specific recommendations, and they unfortunately do not have any data for the protein in ELISA so I have removed this as a tested application.I would still expect this protein to be detected by antibodies diluted in either of the buffers that you mentioned. I would recommend trying a couple of assays to determine the source of the problem: 1) Run a quick dot blot to make sureeach individual antibody reacts with the protein. We have a protocol for this kind of assay- https://www.abcam.com/index.html?pageconfig=resource&rid=11452 If the antibodies individually react with the protein, but there is no signal in the sandwich, the binding sites of the antibodies might interfere with each other in some way. Since the conformation of this recombinant protein could be different than the native conformation of the protein in your samples, this won't necessarily be seen with your samples. If you do find that this is the case, you can try a different pair of antibodies, though I realize you've already developed the sandwich pair with these particular antibodies. If you can find a protein that has been isolated from native tissue, it may also work better in this particular assay. 2) The protein should be at the stated concentration, but itwould be a good idea to check the concentration of the working dilutions by Nanodrop or BCA assay, etc. As we discussed, I would be happy to send another vial of the protein so you can continue to troubleshoot. Please let me know your order number or PO number if this is something that you would be interested in, and I will send that to you promptly.

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