Recombinant Human Creatine kinase MT protein (ab128427)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human Creatine kinase MT protein -
Purity
> 95 % SDS-PAGE.
ab128427 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH -
Predicted molecular weight
45 kDa including tags -
Amino acids
40 to 417 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab128427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
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Alternative names
- Acidic type mitochondrial creatine kinase
- CKMT
- CKMT1
see all -
Relevance
Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. -
Cellular localization
Cell Membrane and Mitochondrial
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab128427 has not yet been referenced specifically in any publications.