Recombinant Human Creatine kinase MT protein (ab128427)

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SDS-PAGE - Recombinant Human Creatine kinase MT protein (ab128427)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human Creatine kinase MT protein

    • Purity

      > 95 % SDS-PAGE.
      ab128427 was purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P12532

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH

      • Predicted molecular weight

        45 kDa including tags

      • Amino acids

        40 to 417

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab128427 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • Acidic type mitochondrial creatine kinase
      • CKMT
      • CKMT1
      • CKMT1A
      • CKMT1B
      • Creatine kinase mitochondrial 1 ubiquitous
      • Creatine kinase U type, mitochondrial
      • Creatine kinase ubiquitous mitochondrial
      • Creatine kinase, mitochondrial 1 (ubiquitous)
      • Creatine kinase, mitochondrial 1A
      • Creatine kinase, mitochondrial 1B
      • Mia CK
      • U MtCK
      • Ubiquitous mitochondrial creatine kinase
      • UMTCK
      see all

    • Relevance

      Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.

    • Cellular localization

      Cell Membrane and Mitochondrial

    Images

    • SDS-PAGE - Recombinant Human Creatine kinase MT protein (ab128427)
      SDS-PAGE - Recombinant Human Creatine kinase MT protein (ab128427)
      15% SDS-PAGE analysis of 3µg ab128427.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab128427? Please let us know so that we can cite the reference in this datasheet.

    ab128427 has not yet been referenced specifically in any publications.

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