Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human CREBBP protein
See all CREBBP proteins and peptides
Purity> 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGS NSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPT PDPAALKDR RMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQK ELEEKRRSRLHKQGILGNQ PALPAPGAQPPVIPQAQPVRPPNGPLSLP VNRMQVSQGMNSFNPMSLGNVQLPQAPMG PRAASPMNHSVQMNSMGSV PGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQ FPSSSGAMS VGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPT PPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGS VPSATQTQS TPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVH AQPPGTPLSQAAASIDNRV PTPSSVASAETNSQQPGPDVPVLEMKTET QAEDTEPDPGESKGEPRSEMMEEDLQGAS QVKEETDIAEQKSEPMEVD EKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPE ELRQALMPT LEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQ YQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQS LGYCCGRKY EFS
Predicted molecular weight51 kDa
Amino acids518 to 1207
Our Abpromise guarantee covers the use of ab135297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
- CREB binding protein
FunctionAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
Involvement in diseaseNote=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
Sequence similaritiesContains 1 bromo domain.
Contains 1 KIX domain.
Contains 2 TAZ-type zinc fingers.
Contains 1 ZZ-type zinc finger.
DomainThe KIX domain mediates binding to HIV-1 Tat.
modificationsMethylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
Cellular localizationCytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab135297 has been referenced in 1 publication.
- Piccirillo AR et al. Histone acetyltransferase CBP is critical for conventional effector and memory T-cell differentiation in mice. J Biol Chem 294:2397-2406 (2019). PubMed: 30573679