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In regards to ab96279, can this be used in zebrafish? I am trying to find CRH expression in the zebrafish brain and was considering in situ, but have now started exploring antibodies. How should I go about it, and can I use any CRH antibody in the zebrafish?
Asked on Mar 12 2012
Thank you for contacting us with your question about our CRH antibodies.
The product ab96279 is actually a recombinant CRH protein, so it could be used as a positive control in a Western blot or ELISAbut it can't be used solely for detection. I've looked through our CRH antibodies, and ab80360 and ab11133 are expected to have the highest reactivity with zebrafish protein (the immunogens are 94% and 80% identical to the zebrafish CRH, respectively).
As we haven't tested either of these antibodies with zebrafish, we can't guarantee them to work, however we do have a testing discount program that you might be interested in. For this program, you would purchase the CRH antibody and test it in your assay, then submit your data to us as an Abreview. In exchange, we'll give you one free primary antibody on a future order. I also noticed that ab80360 has only been tested in Western blot while ab11133 has only been tested in immunohistochemistry on frozen tissue sections (IHC-Fr). Which application would you be using the antibody in?
Since ab80360 is raised in chicken, you will need to use an anti-chicken seconday antibody such as ab6753-
Ab11133 is raised in rabbit so it can be used with an anti-rabbit secondary antibody such as ab6721-
I hope this information will be useful. Please let me know if you need any assistance setting up your experiment or if you would like more information about our testing discount program. I will be happy to help further.
Answered on Mar 12 2012