Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human CSF-1-R protein (Active)
See all CSF-1-R proteins and peptides
Specific activity = 24 pmol/min/μg.
Assay buffer: 5 mM MOPS, 2.5 mM glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate with 0.25 mg/ml Poly (Glu:Tyr, 4:1) peptide substrate and [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Purity>= 80 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceYKYKQKPKYQ VRWKIIESYE GNSYTFIDPT QLPYNEKWEF PRNNLQFGKT LGAGAFGKVV EATAFGLGKE DAVLKVAVKM LKSTAHADEK EALMSELKIM SHLGQHENIV NLLGACTHGG PVLVITEYCC YGDLLNFLRR KAEAMLGPSL SPGQDPEGGV DYKNIHLEKK YVRRDSGFSS QGVDTYVEMR PVSTSSNDSF SEQDLDKEDG RPLELRDLLH FSSQVAQGMA FLASKNCIHR DVAARNVLLT NGHVAKIGDF GLARDIMNDS NYIVKGNARL PVKWMAPESI FDCVYTVQSD VWSYGILLWE IFSLGLNPYP GILVNSKFYK LVKDGYQMAQ PAFAPKNIYS IMQACWALEP THRPTFQQIC SFLQEQAQED RRERDYTNLP SSSRSGGSGS SSSELEEESS SEHLTCCEQG DIAQPLLQPN NYQFC
Predicted molecular weight75 kDa including tags
Amino acids538 to 972
TagsGST tag N-Terminus
Additional sequence informationGenbank: BC047521
Our Abpromise guarantee covers the use of ab271478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- C FMS
- CD 115
FunctionProtein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
Tissue specificityExpressed in bone marrow and in differentiated blood mononuclear cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271478 has not yet been referenced specifically in any publications.