Recombinant human CSK protein (ab60764)

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Functional Studies - Recombinant human CSK protein (ab60764)
  • SDS-PAGE - Recombinant human CSK protein (ab60764)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CSK protein
    See all CSK proteins and peptides

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • C SRC
    • C SRC kinase
    • C src Tyrosine Kinase
    • C-SRC kinase
    • c-src tyrosine kinase
    • CSK
    • Csk A
    • CSK_HUMAN
    • CYTOPLASMIC TYROSINE KINASE
    • EC 2.7.10.2
    • MGC112926
    • MGC117393
    • MGC154049
    • P60 Src
    • Protein tyrosine kinase CYL
    • Protein-tyrosine kinase CYL
    • Proto oncogene tyrosine protein kinase
    • Tyrosine protein kinase CSK
    • Tyrosine-protein kinase CSK
    • zgc:154049
    see all

  • Function

    Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

  • Tissue specificity

    Expressed in lung and macrophages.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.

  • Cellular localization

    Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human CSK protein (ab60764)
    Functional Studies - Recombinant human CSK protein (ab60764)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human CSK protein (ab60764)
    SDS-PAGE - Recombinant human CSK protein (ab60764)
    ab60764 on SDS-PAGE, MW ~52kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab60764? Please let us know so that we can cite the reference in this datasheet.

ab60764 has not yet been referenced specifically in any publications.

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