Recombinant human CSK protein (Active) (ab268428)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CSK protein (Active)
See all CSK proteins and peptides -
Biological activity
The specific activity of ab268428 was 365 nmol/min/mg in a kinase assay using Poly (Glu4, Tyr1) as substrate.
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Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
~75 kDa by SDS-PAGE -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank: NM_004383
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab268428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% DTT, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- C SRC
- C SRC kinase
- C src Tyrosine Kinase
see all -
Function
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. -
Tissue specificity
Expressed in lung and macrophages. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. -
Cellular localization
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268428 has not yet been referenced specifically in any publications.