Description

  • Product name

    Recombinant human CSNK2A2 protein
    See all CSNK2A2 proteins and peptides
  • Biological activity

    Activity: > 100.000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide RRRDDDSDDD per min at 37oC).
  • Purity

    > 95 % SDS-PAGE.
    Purity is > 99% by SDS-PAGE.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab90035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as CKII alpha prime polypeptide

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.0308% DTT, 0.316% Tris HCl, 0.0292% EDTA, 1.16% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Casein kinase 2 alpha prime polypeptide
    • Casein kinase II alpha' chain
    • Casein kinase II subunit alpha'
    • CK II
    • CK II alpha'
    • CK2A2
    • CKII
    • CSK22_HUMAN
    • CSNK2A1
    • CSNK2A2
    • FLJ43934
    see all
  • Function

    Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

References

ab90035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The endogenous enzyme is a heterotetramer consisting of 2α/2β subunits. However, I can confirm that ab90035 consists of just the α' subunit of CK2. It's molecular weight of 84.3 kDa is due to a N-MBP fusion tag.

We did not perform in house phosphorylation tests with this protein and therefore we have no data to confirm or guarantee how well it will work for investigating phosphorylation of putative substrates for CKII or a suggested protocol. As far as I am aware recombinant alpha prime subunits in general are suitable for in vitro phosphorylation assays, though we cannot guarantee this without further testing.

I will be pleased to provide an AbTrial discount for you to test ab90035 in your studies in return for some feedback and data in the form of an Abreview. In order to proceed, please following the procedure below:

1. Purchase the product at regular full price and forward the order number or PO back to me by reply email. Please provide more details of the experiment you are planning to perform.
Please note the product to be tested must be purchased at full list price with no other promotions or use of a previous AbTrial code.

2. A personal discount code valued at the purchase price of the product will then be sent to you by email.

3. Submit the results via our online Abreview system, including the discount code in the additional notes section of the Abreview.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

4. Once the Abreview is submitted, the discount code will become active.

5. Apply the discount code on your next order to receive that value off. The purchase must be made within 6 months of the code being issued.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.


I am sorry that we do not have more data to help answer your question more fully in this case. If you have any further queries, please do not hesitate to contact me.

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