Recombinant human CST6 protein (ab211317)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, MS
Description
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Product name
Recombinant human CST6 protein
See all CST6 proteins and peptides -
Biological activity
The IC50 value is < 10nM. The inhibitory function of CST6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.
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Purity
> 90 % SDS-PAGE.
ab211317 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMRPQERMVGELRDLSPDDPQVQKAAQAAVA SYNMGSNSIYYFRDTHIIKAQSQLVAGIKYFLTMEMGSTDCRKTRVTGDH VDLTTCPLAAGAQQEKLRCDFEVLVVPWQNSSQLLKHNCVQM -
Predicted molecular weight
16 kDa including tags -
Amino acids
29 to 149 -
Tags
His tag N-Terminus -
Additional sequence information
This product is for the mature full length protein. The signal peptide and propeptide are not included. NP_001314.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab211317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTT, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CST 6
- CST6
- Cystatin 6
see all -
Function
Shows moderate inhibition of cathepsin B but is not active against cathepsin C. -
Tissue specificity
Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity. -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsSubstrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant human CST6 protein (ab211317)
15% SDS-PAGE analysis of ab211317 (3µg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab211317 has not yet been referenced specifically in any publications.
