Recombinant human CST6 protein (ab211317)

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Description

  • Product name

    Recombinant human CST6 protein
    See all CST6 proteins and peptides

  • Biological activity

    The IC50 value is < 10nM. The inhibitory function of CST6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

  • Purity

    > 90 % SDS-PAGE.
    ab211317 was purified using conventional chromatography techniques.

  • Expression system

    Escherichia coli

  • Accession

    Q15828

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMRPQERMVGELRDLSPDDPQVQKAAQAAVA SYNMGSNSIYYFRDTHIIKAQSQLVAGIKYFLTMEMGSTDCRKTRVTGDH VDLTTCPLAAGAQQEKLRCDFEVLVVPWQNSSQLLKHNCVQM

    • Predicted molecular weight

      16 kDa including tags

    • Amino acids

      29 to 149

    • Tags

      His tag N-Terminus

    • Additional sequence information

      This product is for the mature full length protein. The signal peptide and propeptide are not included. NP_001314.

Specifications

Our Abpromise guarantee covers the use of ab211317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CST 6
    • CST6
    • Cystatin 6
    • Cystatin E
    • Cystatin E/M
    • Cystatin M
    • Cystatin M/E
    • Cystatin-6
    • Cystatin-E
    • Cystatin-M
    • Cystatin6
    • CystatinE
    • CystatinM
    • Cysteine proteinase inhibitor
    • CYTM_HUMAN
    see all

  • Function

    Shows moderate inhibition of cathepsin B but is not active against cathepsin C.

  • Tissue specificity

    Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.

  • Sequence similarities

    Belongs to the cystatin family.

  • Post-translational
    modifications

    Substrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human CST6 protein (ab211317)
    SDS-PAGE - Recombinant human CST6 protein (ab211317)

    15% SDS-PAGE analysis of ab211317 (3µg).   

References

ab211317 has not yet been referenced specifically in any publications.

Publishing research using ab211317? Please let us know so that we can cite the reference in this datasheet.

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