Recombinant Human CTNNA1 protein (Tagged) (ab51443)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human CTNNA1 protein (Tagged) -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
123 kDa including tags -
Tags
GST tag N-Terminus -
Additional sequence information
NM 001903
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Specifications
Our Abpromise guarantee covers the use of ab51443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
This product was previously labelled as alpha 1 Catenin
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- Alpha E-catenin
- Cadherin associated protein
- Cadherin associated protein 102kDa
see all -
Function
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. -
Tissue specificity
Expressed ubiquitously in normal tissues. -
Sequence similarities
Belongs to the vinculin/alpha-catenin family. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane and Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CTNNA1 protein (Tagged) (ab51443)
SDS-PAGE analysis of recombinant human CTNNA1 protein (Tagged) (ab51443) .
MW ~123 kDa
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Western blot - Recombinant Human CTNNA1 protein (Tagged) (ab51443)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab51443 has been referenced in 1 publication.
- Yosefzon Y et al. Caspase-3 Regulates YAP-Dependent Cell Proliferation and Organ Size. Mol Cell 70:573-587.e4 (2018). Functional Studies . PubMed: 29775577
