Recombinant Human Cullin 2/CUL-2 protein (Tagged) (ab271483)

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Description

  • Product name

    Recombinant Human Cullin 2/CUL-2 protein (Tagged)
    See all Cullin 2/CUL-2 proteins and peptides

  • Purity

    >= 45 % SDS-PAGE.

  • Expression system

    HEK 293 cells

  • Accession

    NM_003591

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SLKPRVVDFD ETWNKLLTTI KAVVMLEYVE RATWNDRFSD IYALCVAYPE PLGERLYTET KIFLENHVRH LHKRVLESEE QVLVMYHRYW EEYSKGADYM DCLYRYLNTQ FIKKNKLTEA DLQYGYGGVD MNEPLMEIGE LALDMWRKLM VEPLQAILIR MLLREIKNDR GGEDPNQKVI HGVINSFVHV EQYKKKFPLK FYQEIFESPF LTETGEYYKQ EASNLLQESN CSQYMEKVLG RLKDEEIRCR KYLHPSSYTK VIHECQQRMV ADHLQFLHAE CHNIIRQEKK NDMANMYVLL RAVSTGLPHM IQELQNHIHD EGLRATSNLT QENMPTLFVE SVLEVHGKFV QLINTVLNGD QHFMSALDKA LTSVVNYREP KSVCKAPELL AKYCDNLLKK SAKGMTENEV EDRLTSFITV FKYIDDKDVF QKFYARMLAK RLIHGLSMSM DSEEAMINKL KQACGYEFTS KLHRMYTDMS VSADLNNKFN NFIKNQDTVI DLGISFQIYV LQAGAWPLTQ APSSTFAIPQ ELEKSVQMFE LFYSQHFSGR KLTWLHYLCT GEVKMNYLGK PYVAMVTTYQ MAVLLAFNNS ETVSYKELQD STQMNEKELT KTIKSLLDVK MINHDSEKED IDAESSFSLN MNFSSKRTKF KITTSMQKDT PQEMEQTRSA VDEDRKMYLQ AAIVRIMKAR KVLRHNALIQ EVISQSRARF NPSISMIKKC IEVLIDKQYI ERSQASADEY SYVA

    • Predicted molecular weight

      88 kDa including tags

    • Amino acids

      2 to 745

    • Tags

      DDDDK tag N-Terminus

    • Additional sequence information

      Genbank: NM_003591

Specifications

Our Abpromise guarantee covers the use of ab271483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Do Not Freeze.

    pH: 8.0
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol

General Info

  • Alternative names

    • Cul2
    • CUL 2
    • CUL-2
    • CUL2
    • CUL2_HUMAN
    • cullin 2
    • cullin homolog 2
    • Cullin-2
    • MGC131970
    see all

  • Function

    Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the cullin family.

  • Post-translational
    modifications

    CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Cullin 2/CUL-2 protein (Tagged) (ab271483)
    SDS-PAGE - Recombinant Human Cullin 2/CUL-2 protein (Tagged) (ab271483)

    SDS-PAGE analysis of ab271483.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab271483? Please let us know so that we can cite the reference in this datasheet.

ab271483 has not yet been referenced specifically in any publications.

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