Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      Recombinant Human NAP-2 is a 7.6 kDa protein containing 70 amino acid residues: AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KD GRKICLDP DAPRIKKIVQKKLAGDESAD

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Form
    Lyophilised
  • Additional notes

    Previously labelled as CXCL7. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

General Info

  • Alternative names
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • Beta-TG
    • C-X-C motif chemokine 7
    • Chemokine (C X C motif) ligand 7
    • Connective tissue activating peptide III
    • CTAP 3
    • CTAP III
    • CTAP-III
    • CTAP-III(1-81)
    • CTAP3
    • CTAPIII
    • CXC chemokine ligand 7
    • CXCL 7
    • CXCL7
    • CXCL7_HUMAN
    • LA PF 4
    • LA-PF4
    • LDGF
    • Leukocyte derived growth factor
    • Leukocyte-derived growth factor
    • Low-affinity platelet factor IV
    • Macrophage-derived growth factor
    • MDGF
    • NAP 2
    • NAP-2
    • NAP-2(1-63)
    • NAP-2(1-66)
    • NAP-2(73)
    • NAP-2(74)
    • Neutrophil activating peptide 2
    • Neutrophil-activating peptide 2(1-63)
    • PBP
    • Platelet basic protein
    • PPBP
    • Pro platelet basic protein
    • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine subfamily B member 7
    • Small-inducible cytokine B7
    • TC1
    • TC2
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thromboglobulin, beta-1
    see all
  • Function
    LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
    Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
    NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
    NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Recombinant Human CXCL7 / PBP protein (ab9556) at 1/1000 dilution + Human CXCL7 active recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 11 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and Diluting buffer - 5% NFDM/TBST

References

ab9556 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry.

For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a concentration of 5.0 - 10.0 ug/ml of this antibody was required. This may need some individual optimization.

The recombinant protein used in this assay is NAP-2 protein ab9556.
https://www.abcam.com/index.html?datasheet=9556 (or use the following: https://www.abcam.com/index.html?datasheet=9556).

For ab9679, Rabbit Anti Human RANTES, this has been tested in Neutralisation as follows:
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 0.4 – 0.6 μg/ml of this antibodywas required. This may need some individual optimization.

The recombinant protein used in this assay, is Rantes protein ab9680.
https://www.abcam.com/index.html?datasheet=9680 (or use the following: https://www.abcam.com/index.html?datasheet=9680).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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