Recombinant Human CYB5R1 protein (ab156728)

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SDS-PAGE - Recombinant Human CYB5R1 protein (ab156728)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human CYB5R1 protein

    • Purity

      > 85 % SDS-PAGE.
      ab156728 is purified using conventional chromatography techniques (anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA).

    • Expression system

      Escherichia coli

    • Accession

      Q9UHQ9

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSLVRRSRR PQVTLLDPNE KYLLRLLDKT TVSHNTKRFR FALPTAHHTL GLPVGKHIYL STRIDGSLVI RPYTPVTSDE DQGYVDLVIK VYLKGVHPKF PEGGKMSQYL DSLKVGDVVE FRGPSGLLTY TGKGHFNIQP NKKSPPEPRV AKKLGMIAGG TGITPMLQLI RAILKVPEDP TQCFLLFANQ TEKDIILRED LEELQARYPN RFKLWFTLDH PPKDWAYSKG FVTADMIREH LPAPGDDVLV LLCGPPPMVQ LACHPNLDKL GYSQKMRFTY

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        29 to 305

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab156728 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • b5R.1
      • B5R1
      • B5R2
      • CYB5R1
      • Cytochrome b5 reductase 1
      • EC 1.6.2.2
      • Humb5R2
      • NAD
      • NAD(P)H:quinone oxidoreductase type 3 polypeptide A2
      • NAD(P)H:quinone oxidoreductase type 3, polypeptide A2
      • NADH cytochrome b5 reductase 1
      • NADH-cytochrome b5 reductase 1
      • NB5R1_HUMAN
      • NQO3A2
      • UNQ3049/PRO9865
      see all

    • Function

      NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

    • Tissue specificity

      Widely expressed.

    • Sequence similarities

      Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
      Contains 1 FAD-binding FR-type domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CYB5R1 protein (ab156728)
      SDS-PAGE - Recombinant Human CYB5R1 protein (ab156728)
      15% SDS-PAGE analysis of ab156728 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab156728? Please let us know so that we can cite the reference in this datasheet.

    ab156728 has not yet been referenced specifically in any publications.

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