Description

  • Product name

    Recombinant Human CYB5R3 protein
  • Purity

    > 95 % SDS-PAGE.
    ab119468 was purified using conventional chromatography.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF
    • Predicted molecular weight

      34 kDa including tags
    • Amino acids

      27 to 301
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab119468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • B5R
    • Cyb5r3
    • Cytochrome b5 reductase
    • Cytochrome b5 reductase 3
    • DIA1
    • Diaphorase 1
    • Diaphorase-1
    • NADH cytochrome b5 reductase 3
    • NADH-cytochrome b5 reductase 3 membrane-bound form
    • NADH-cytochrome b5 reductase 3 soluble form
    • NB5R3_HUMAN
    • OTTHUMP00000028761
    • OTTHUMP00000198435
    • OTTHUMP00000198574
    • OTTHUMP00000198662
    • OTTHUMP00000198665
    see all
  • Function

    Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
  • Tissue specificity

    Isoform 2 is expressed at late stages of erythroid maturation.
  • Involvement in disease

    Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
  • Sequence similarities

    Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab119468 (3µg)

References

ab119468 has not yet been referenced specifically in any publications.

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