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    recombinant-human-cyclin-b1-protein-ab128445.pdf

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Cell Biology Cell Cycle Cyclins
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Recombinant Human Cyclin B1 protein (ab128445)

  • Datasheet
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SDS-PAGE - Recombinant Human Cyclin B1 protein (ab128445)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human Cyclin B1 protein
    • Purity

      > 85 % SDS-PAGE.
      ab128445 was purified using conventional chromatography.
    • Expression system

      Escherichia coli
    • Accession

      P14635
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSHMALRVT RNSKINAENK AKINMAGAKR VPTAPAATSK PGLRPRTALG DIGNKVSEQL QAKMPMKKEA KPSATGKVID KKLPKPLEKV PMLVPVPVSE PVPEPEPEPE PEPVKEEKLS PEPILVDTAS PSPMETSGCA PAEEDLCQAF SDVILAVNDV DAEDGADPNL CSEYVKDIYA YLRQLEEEQA VRPKYLLGRE VTGNMRAILI DWLVQVQMKF RLLQETMYMT VSIIDRFMQN NCVPKKMLQL VGVTAMFIAS KYEEMYPPEI GDFAFVTDNT YTKHQIRQME MKILRALNFG LGRPLPLHFL RRASKIGEVD VEQHTLAKYL MELTMLDYDM VHFPPSQIAA GAFCLALKIL DNGEWTPTLQ HYLSYTEESL LPVMQHLAKN VVMVNQGLTK HMTVKNKYAT SKHAKISTLP QLNSALVQDL AKAVAKV
      • Predicted molecular weight

        51 kDa including tags
      • Amino acids

        1 to 433
      • Tags

        His tag N-Terminus

    Associated products

    • Positive Controls

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab128445 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • CCNB
      • CCNB 1
      • ccnb1
      • CCNB1_HUMAN
      • Cyclin B1
      • G2 mitotic specific cyclin B1
      • G2/mitotic-specific cyclin-B1
      see all
    • Function

      Essential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similarities

      Belongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stage

      Accumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications

      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Target information above from: UniProt accession P14635 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Cyclin B1 protein (ab128445)
      SDS-PAGE - Recombinant Human Cyclin B1 protein (ab128445)

      SDS-PAGE analysis of ab128445 (3 µg) under reducing conditions and visualized by coomassie blue stain.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab128445? Please let us know so that we can cite the reference in this datasheet.

    ab128445 has been referenced in 3 publications.

    • Steingruber M  et al. Cyclins B1, T1, and H differ in their molecular mode of interaction with cytomegalovirus protein kinase pUL97. J Biol Chem 294:6188-6203 (2019). PubMed: 30782840
    • Daldello EM  et al. Cyclin B2 is required for progression through meiosis in mouse oocytes. Development 146:N/A (2019). PubMed: 30952665
    • Steingruber M  et al. Proteomic Interaction Patterns between Human Cyclins, the Cyclin-Dependent Kinase Ortholog pUL97 and Additional Cytomegalovirus Proteins. Viruses 8:N/A (2016). PubMed: 27548200

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