Recombinant Human Cyclin B1 protein (ab128445)

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Description

  • Product name

    Recombinant Human Cyclin B1 protein

  • Purity

    > 80 % SDS-PAGE.
    ab128445 was purified using conventional chromatography.

  • Expression system

    Escherichia coli

  • Accession

    P14635

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMALRVT RNSKINAENK AKINMAGAKR VPTAPAATSK PGLRPRTALG DIGNKVSEQL QAKMPMKKEA KPSATGKVID KKLPKPLEKV PMLVPVPVSE PVPEPEPEPE PEPVKEEKLS PEPILVDTAS PSPMETSGCA PAEEDLCQAF SDVILAVNDV DAEDGADPNL CSEYVKDIYA YLRQLEEEQA VRPKYLLGRE VTGNMRAILI DWLVQVQMKF RLLQETMYMT VSIIDRFMQN NCVPKKMLQL VGVTAMFIAS KYEEMYPPEI GDFAFVTDNT YTKHQIRQME MKILRALNFG LGRPLPLHFL RRASKIGEVD VEQHTLAKYL MELTMLDYDM VHFPPSQIAA GAFCLALKIL DNGEWTPTLQ HYLSYTEESL LPVMQHLAKN VVMVNQGLTK HMTVKNKYAT SKHAKISTLP QLNSALVQDL AKAVAKV

    • Predicted molecular weight

      51 kDa including tags

    • Amino acids

      1 to 433

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab128445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • CCNB
    • CCNB 1
    • ccnb1
    • CCNB1_HUMAN
    • Cyclin B1
    • G2 mitotic specific cyclin B1
    • G2/mitotic-specific cyclin-B1
    see all

  • Function

    Essential for the control of the cell cycle at the G2/M (mitosis) transition.

  • Sequence similarities

    Belongs to the cyclin family. Cyclin AB subfamily.

  • Developmental stage

    Accumulates steadily during G2 and is abruptly destroyed at mitosis.

  • Post-translational
    modifications

    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Cyclin B1 protein (ab128445)
    SDS-PAGE - Recombinant Human Cyclin B1 protein (ab128445)
    15% SDS-PAGE analysis of ab128445 (3µg).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab128445? Please let us know so that we can cite the reference in this datasheet.

ab128445 has been referenced in 3 publications.

  • Steingruber M  et al. Cyclins B1, T1, and H differ in their molecular mode of interaction with cytomegalovirus protein kinase pUL97. J Biol Chem 294:6188-6203 (2019). PubMed: 30782840
  • Daldello EM  et al. Cyclin B2 is required for progression through meiosis in mouse oocytes. Development 146:N/A (2019). PubMed: 30952665
  • Steingruber M  et al. Proteomic Interaction Patterns between Human Cyclins, the Cyclin-Dependent Kinase Ortholog pUL97 and Additional Cytomegalovirus Proteins. Viruses 8:N/A (2016). PubMed: 27548200

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