Recombinant human Cyclin E1 + Cdk3 protein (Active) (ab196131)

Description

  • Product name

    Recombinant human Cyclin E1 + Cdk3 protein (Active)
  • Biological activity

    Specific activity of ab196131 was determined to be 30 pmol/min/μg. Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/mL) substrate and 20 µM ATP at 30°C for 50 min.

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Predicted molecular weight

      60 kDa
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      Recombinant full length human Cdk3 (NM_001258)
    • Amino Acid Sequence 2

    • Species

      Human
    • Predicted molecular weight

      73 kDa
    • Additional sequence information

      Recombinant full length Cyclin E1 (NM_001238)

Specifications

Our Abpromise guarantee covers the use of ab196131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    This is a complex of Cdk3 and Cyclin E1 Co-expressed in a Baculovirus-infected Sf9 cell expression system.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.12% Glutathione, 0.0462% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localization

    Cyclin E1: Nucleus.

Images

  • Specific activity of ab196131 was determined to be 30 pmol/min/μg.

  • 10% SDS-PAGE analysis of ab196131 stained with Coomassie Blue.

    Lane 1: Cdk3 Lower band and Cyclin E1 upper band
    Lane 2: Protein Marker

References

ab196131 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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