Recombinant human Cyclin E1 + CDK3 protein (Active) (ab196131)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human Cyclin E1 + CDK3 protein (Active) -
Biological activity
Specific activity of ab196131 was determined to be 30 pmol/min/μg. Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/mL) substrate and 20 µM ATP at 30°C for 50 min.
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Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Predicted molecular weight
60 kDa -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant full length human Cdk3 (NM_001258)
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Amino Acid Sequence 2
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Species
Human -
Predicted molecular weight
73 kDa -
Additional sequence information
Recombinant full length Cyclin E1 (NM_001238)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This is a complex of Cdk3 and Cyclin E1 Co-expressed in a Baculovirus-infected Sf9 cell expression system.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.12% Glutathione, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
Cyclin E1: Nucleus.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab196131 has not yet been referenced specifically in any publications.