Recombinant Human Cyclin Y protein (Tagged) (ab174060)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Tags: proprietary tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human Cyclin Y protein (Tagged)
See all Cyclin Y proteins and peptides -
Purity
> 80 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENID DLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSC STIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHP LSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLT YAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNE LERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHK LEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS -
Predicted molecular weight
65 kDa including tags -
Amino acids
2 to 341 -
Tags
proprietary tag N-Terminus -
Additional sequence information
(NM_145012)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- C10orf9
- CBCP1
- CCNX
see all -
Function
Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the cyclin family. Cyclin Y subfamily.
Contains 1 cyclin N-terminal domain. -
Developmental stage
Enriched at G2/M. -
Post-translational
modificationsUbiquitinated; leading to its degradation.
Heavily phosphorylated. Phosphorylation at Ser-71 and Ser-73 by CDK14 is enhanced during the G2 and M cell cycle phases, and creates a phosphodegron triggering SCF-dependent ubiquitination. -
Cellular localization
Nucleus and Cell membrane. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174060 has not yet been referenced specifically in any publications.