Key features and details
- Expression system: Wheat germ
- Suitable for: ELISA, WB, SDS-PAGE
Product nameRecombinant Human Cytochrome P450 17A1/CYP17A1 protein
Expression systemWheat germ
Protein lengthFull length protein
SequenceMWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLPRHGHMH NNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKGKDFSGRPQMA TLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEI STLCDMLATHNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQN YNEGIIDNLSKDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENY KEKFRSDSITNMLDTLMQAKMNSDNGNAGPDQDSELLSDNHILTTIGDIF GAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRL LLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALH HNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQ ELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWR EAQAEGST
Predicted molecular weight82 kDa including tags
Amino acids1 to 508
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [OTI5G10] (ab118988)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6294(2)] (ab134910)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab48019)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206)
Our Abpromise guarantee covers the use of ab152320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Cytochrome P450 17A1.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- 20 lyase
FunctionConversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
PathwayLipid metabolism; steroid biosynthesis.
Involvement in diseaseDefects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic).
Sequence similaritiesBelongs to the cytochrome P450 family.
modificationsPhosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab152320 has not yet been referenced specifically in any publications.