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Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)

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SDS-PAGE - Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, ELISA, SDS-PAGE

    Description

    • Product name

      Recombinant Human Cytochrome P450 3A4/CYP3A4 protein
      See all Cytochrome P450 3A4/CYP3A4 proteins and peptides
    • Expression system

      Wheat germ
    • Accession

      P08684
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        DPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMK LALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA
      • Predicted molecular weight

        37 kDa including tags
      • Amino acids

        404 to 503

    Specifications

    Our Abpromise guarantee covers the use of ab114328 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      This product was previously labelled as Cytochrome P450 3A4.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 1,8-cineole 2-exo-monooxygenase
      • Albendazole monooxygenase
      • Albendazole sulfoxidase
      • CP33
      • CP34
      • CP3A4_HUMAN
      • CYP3
      • CYP3A
      • CYP3A3
      • CYP3A4
      • CYPIIIA3
      • CYPIIIA4
      • Cytochrome P450 3A3
      • Cytochrome P450 3A4
      • Cytochrome P450 family 3 subfamily A polypeptide 4
      • Cytochrome P450 HLp
      • Cytochrome P450 NF-25
      • Cytochrome P450 subfamily IIIA polypeptide 4
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3
      • cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
      • Cytochrome P450-PCN1
      • Glucocorticoid inducible P450
      • HLP
      • MGC126680
      • NF 25
      • NF25
      • Nifedipine oxidase
      • P450 III steroid inducible
      • P450 PCN1
      • P450, family III
      • P450C3
      • P450PCN1
      • Quinine 3 monooxygenase
      • Quinine 3-monooxygenase
      • Taurochenodeoxycholate 6 alpha hydroxylase
      • Taurochenodeoxycholate 6-alpha-hydroxylase
      see all
    • Function

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
    • Tissue specificity

      Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Post-translational
      modifications

      Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Target information above from: UniProt accession P08684 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)
      SDS-PAGE - Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)
      ab114328 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab114328? Please let us know so that we can cite the reference in this datasheet.

    ab114328 has not yet been referenced specifically in any publications.

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