Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)
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Description
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Product name
Recombinant Human Cytochrome P450 3A4/CYP3A4 protein
See all Cytochrome P450 3A4/CYP3A4 proteins and peptides -
Expression system
Wheat germ -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DPKYWTEPEKFLPERFSKKNKDNIDPYIYTPFGSGPRNCIGMRFALMNMK LALIRVLQNFSFKPCKETQIPLKLSLGGLLQPEKPVVLKVESRDGTVSGA -
Predicted molecular weight
37 kDa including tags -
Amino acids
404 to 503
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Specifications
Our Abpromise guarantee covers the use of ab114328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as Cytochrome P450 3A4.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.3% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 1,8-cineole 2-exo-monooxygenase
- Albendazole monooxygenase
- Albendazole sulfoxidase
see all -
Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. -
Tissue specificity
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPolyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Cytochrome P450 3A4/CYP3A4 protein (ab114328)ab114328 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
Datasheets and documents
References
ab114328 has not yet been referenced specifically in any publications.
