Product nameRecombinant Human Cytokeratin 18 protein
See all Cytokeratin 18 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
Molecular weight48 kDa
- Anti-Cytokeratin 18 antibody [E431-1] (ab32118)
- Anti-Cytokeratin 18 antibody (ab52948)
- Anti-Cytokeratin 18 antibody [C-04] (ab668)
- Anti-Cytokeratin 18 antibody [DA-7] (ab669)
- Anti-Cytokeratin 18 antibody [DC 10] (ab7797)
- Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
- Anti-Cytokeratin 18 antibody [DEK-18] (ab9387)
Our Abpromise guarantee covers the use of ab73638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity> 95 % SDS-PAGE.
ab73638 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Additional notesReconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.068% Methylamine hydrochloride, 0.0584% EDTA, 0.474% Tris HCl, 9.5% Urea
ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
- Cell proliferation inducing gene 46 protein
- Cell proliferation inducing protein 46
- Cell proliferation-inducing gene 46 protein
FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
ab73638 has not yet been referenced specifically in any publications.