Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      48 kDa

Specifications

Our Abpromise guarantee covers the use of ab73638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    ab73638 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • Form
    Lyophilised
  • Additional notes
    Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.068% Methylamine hydrochloride, 0.0584% EDTA, 0.474% Tris HCl, 9.5% Urea

  • Reconstitution
    Reconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • Cell proliferation inducing gene 46 protein
    • Cell proliferation inducing protein 46
    • Cell proliferation-inducing gene 46 protein
    • CK 18
    • CK-18
    • CK18
    • CYK 18
    • CYK18
    • Cytokeratin 18
    • Cytokeratin endo B
    • Cytokeratin-18
    • K 18
    • K18
    • K1C18_HUMAN
    • KA18
    • Keratin 18
    • Keratin 18, type I
    • Keratin D
    • keratin, type I cytoskeletal 18
    • Keratin-18
    • Krt18
    see all
  • Function
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificity
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in disease
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt

Images

References

ab73638 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. Some of our products are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within six month of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products. As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product. I am very pleased to send a CoC. It is attached to this email. We do not have set expiration dates for our products. Most lyophilized proteins are stable and can last up to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the product you purchased. These conditions will vary among our products, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least six month from the date of purchase when stored correctly.   In the case of ab73638 I suggest to freeze it lyophilized and it should be stable for years. But I have to confirm that our Abpromise  will only guarantee this product for up to six month. I hope this information helps. Please do not hesitate to contact us if you need anything further.  

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