Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Cytokeratin 8 protein
See all Cytokeratin 8 proteins and peptides
Purity> 90 % SDS-PAGE.
ab156970 is purified using conventional chromatography techniques.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMGSSHHHHHHSSGLVPRGSHMGSMSIRVTQKSYKVSTSGPRAFSSRSYTS GPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLV LEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQ QQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKN KYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQL YEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAE AESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQ RASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQEL MNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAY GGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSES SDVLPK
Predicted molecular weight56 kDa including tags
Amino acids1 to 483
TagsHis tag N-Terminus
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Cytokeratin 8 antibody [C-43] (ab2530)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
- Anti-Cytokeratin 8 antibody (ab59400)
- Anti-Cytokeratin 8 antibody [35bH11] (ab758)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab156970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol
- CK 8
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab156970 has been referenced in 1 publication.
- Goldie BJ et al. miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif. Front Mol Neurosci 10:259 (2017). PubMed: 28878619