Key features and details
- Expression system: Baculovirus
- Purity: >= 87% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human Cytosolic Phospholipase A2 protein
See all Cytosolic Phospholipase A2 proteins and peptides
Specific Activity: 57 pmol/min/µg.
Assay Conditions: PLA reaction was done using the 1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholine
substrate. Enzyme reaction is conducted in 100 µl volume containing 50 mM Tris pH 8.0, 500 mM NaCl, 10 mM CaCl2, 0.5 mg/ml BSA, 10 µM substrate and 200 ng ab198469. Reaction progress was monitored at ex345/em395 for 10 minutes continuously at room temperature.
Purity>= 87 % SDS-PAGE.
Protein lengthFull length protein
SequenceMSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFI STTPDSRKRTRHFNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDE TLGTATFTVSSMKVGEKKE VPFIFNQVTEMVLEMSLEVCSCPDLRFSM ALCDQEKTFRQQRKEHIRESMKKLLGPKN SEGLHSARDVPVVAILGSG GGFRAMVGFSGVMKALYESGILDCATYVAGLSGSTWYM STLYSHPDFP EKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLWKKKSSGQPVTFTD IFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELM FADWVEFSPYE IGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLM GVWGSAFSILFNRVLGVSGSQ SRGSTMEEELENITTKHIVSNDSSDSD DESHEPKGTENEDAGSDYQSDNQASWIHRMI MALVSDSALFNTREGRA GKVHNFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVAD PDEFERI YEPLDVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSS PPFK ELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEK DCPTIIHFVLANINFR KYKAPGVPRETEEEKEIADFDIFDDPESPFST FNFQYPNQAFKRLHDLMHFNTLNNIDVI KEAMVESIEYRRQNPSRCSV SLSNVEARRFFNKEFLSKPKADYKDDDDKHHHHHH
Predicted molecular weight87 kDa including tags
Amino acids1 to 749
TagsHis-DDDDK tag C-Terminus
Additional sequence informationGenBank Accession No. NM_024420
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)
- Anti-Cytosolic Phospholipase A2 antibody (ab139388)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Cytosolic Phospholipase A2 antibody (ab58375)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Calcium dependent phospholipid binding protein
- CPLA 2
FunctionSelectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Tissue specificityExpressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PLA2c domain.
DomainThe N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
modificationsActivated by phosphorylation at both Ser-505 and Ser-727.
Cellular localizationCytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198469 has not yet been referenced specifically in any publications.