Description

  • Product name

    Recombinant Human Dcp2/TDT protein
  • Expression system

    Wheat germ
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFY MQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTY GAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETG FDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWF SIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSD NGFSSTGSTPAKPTVEKLSRTKFRHSQQLFPDGSPGDQWVKHRQPLQQKP YNNHSEMSDLLKGKKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAE GQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL
    • Predicted molecular weight

      68 kDa including tags
    • Amino acids

      1 to 385

Associated products

Specifications

Our Abpromise guarantee covers the use of ab132368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 µg/µl.

     This product was previously labelled as Dcp2

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • DCP2
    • DCP2 decapping enzyme homolog (S. cerevisiae)
    • DCP2, S. cerevisiae, homolog of
    • DCP2_HUMAN
    • decapping enzyme 2, S. cerevisiae, homolog of
    • decapping enzyme homolog (S. cerevisiae)
    • FLJ33245
    • hDpc
    • m7GpppN-mRNA hydrolase
    • mRNA decapping enzyme 2
    • mRNA-decapping enzyme 2
    • Nucleoside diphosphate-linked moiety X motif 20
    • nudix (nucleoside diphosphate linked moiety X)-type motif 20
    • Nudix motif 20
    • NUDT20
    see all
  • Function

    Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety.
  • Sequence similarities

    Belongs to the Nudix hydrolase family. DCP2 subfamily.
    Contains 1 nudix hydrolase domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > P-body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab132368 stained with Coomassie Blue.

References

ab132368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Other
*1X DCP2 Reaction Buffer:
• 10 mM Tris-HCl, pH 7.5
• 100 mM KCl
• 2 mM MgCl2
• 2 mM DTT
• 0.5 mM MnCl2
*Multiple nudix family proteins possess mRNA decapping activity
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982215/
Decapping conditions:
---------------------------------------------------------------------------------------------------------------------------------
DCP2 **RppH(NeB)
***Substrate 0.1 µg 0.1 µg
RNase inhibitor 40Units 40nits
Decapping reaction buffer 1X 1X(supplied NEB)
Nuclease free-water to 20 μl to 20 μl
DeCapping enzyme 0.05 μg/μ 5Units
---------------------------------------------------------------------------------------------------------------------------------
Incubation Temperature/Time: 37°C, 1 hour.
** Bacterial RNA 5´ Pyrophosphohydrolase (RppH)
***M7Gppp-Spliced leader sequence (40nt) (CAP0)

Mrs. Kathrin Eberhardt

Verified customer

Submitted Mar 08 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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