The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Binds Human and mouse TRAIL.
Inhibits Human soluble TRAIL-mediated apoptosis in a concentration range of 50-100ng/ml.
< 0.100 Eu/µg
>95% by SDS-PAGE .
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute with 50µl sterile water to a final concentration of 1 mg/ml. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Antagonist decoy receptor for TRAIL/Apo 2L
Cytotoxic TRAIL receptor 3
Decoy Receptor 1
Decoy TRAIL receptor without death domain
Lymphocyte inhibitor of TRAIL
TNF related apoptosis inducing ligand receptor 3
TNF-re ated apoptosis inducing ligand receptor 3
TRAIL receptor 3
Tumor necrosis factor receptor superfamily member 10C
Tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.
Cell Membrane and Cytoplasmic
Functional Studies - Recombinant human DcR1 protein (ab157352)
Inhibition of TRAIL-mediated lysis. ab157352 exerts its inhibitory activity in a concentration range of 50-100µg/ml.
Method: Human Jurkat lymphoma cells (50,000 cells in 100µl DMEM medium containing 5% fetal calf serum) were incubated with 0.1µg/ml soluble TRAIL plus 2µg/ml enhancer and increasing concentrations of ab157352 in a 96-well plate for 16 hours at 37°C. Concentrations of ab157352 required to inhibit may vary depending on the cell type studied and on the concentration of sTRAIL used to kill cells. Cell viability was determined using a MTT-based cell proliferation assay kit.
has not yet been referenced specifically in any publications.
Publishing research using ab157352? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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