Recombinant human DCXR protein (Active) (ab219429)
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Description
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Product name
Recombinant human DCXR protein (Active)
See all DCXR proteins and peptides -
Biological activity
Specific activity is > 1,800 pmol/min/ug and is defined as the amount of enzyme that oxidize 1 pmole of xylitol to L-xylulose per minute at pH 10.0 at 37ºC.
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Purity
> 95 % SDS-PAGE.
ab219429 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMELFLAGRRVLVTGAGKGIGRGTVQALHAT GARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLL VNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGA IVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPT VVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMT TGSTLPVEGGFWAC -
Predicted molecular weight
28 kDa including tags -
Amino acids
1 to 244 -
Tags
His tag N-Terminus -
Additional sequence information
NP_057370.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab219429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
Functional Studies
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituents: 20% Glycerol, 0.32% Tris HCl, 0.02% DTT, 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Carbonyl reductase 2
- Carbonyl reductase II
- DCR
see all -
Function
Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. -
Tissue specificity
Highly expressed in kidney, liver and epididymis. In the epididymis, it is mainly expressed in the proximal and distal sections of the corpus region. Weakly or not expressed in brain, lung, heart, spleen and testis. -
Involvement in disease
Note=The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Membrane. Probably recruited to membranes via an interaction with phosphatidylinositol. - Information by UniProt
Images
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SDS-PAGE - Recombinant human DCXR protein (Active) (ab219429)15% SDS-PAGE analysis of 3 μg ab219429.
Datasheets and documents
References
ab219429 has not yet been referenced specifically in any publications.
