Recombinant Human DDAH1 protein (ab99863)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      O94760
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSMAGLGHPAAFGRATHAVVRALPESLGQ HALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFV EDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLD GGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSF CSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNI PNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSV LINKKVDS
    • Molecular weight
      34 kDa including tags
    • Amino acids
      1 to 285
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab99863 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names
    • DDAH
    • DDAH I
    • DDAH-1
    • DDAH1
    • DDAH1_HUMAN
    • DDAHI
    • Dimethylargininase 1
    • Dimethylargininase-1
    • Dimethylarginine dimethylaminohydrolase 1
    • N(G)
    • N(G)-dimethylarginine dimethylaminohydrolase 1
    • NG NG dimethylarginine dimethylaminohydrolase
    see all
  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human DDAH1 protein (ab99863)
    SDS-PAGE - Recombinant Human DDAH1 protein (ab99863)
    15% SDS-PAGE showing ab99863 (3µg).
  • Western blot - Recombinant Human DDAH1 protein (ab99863)
    Western blot - Recombinant Human DDAH1 protein (ab99863)

References

ab99863 has not yet been referenced specifically in any publications.

Publishing research using ab99863? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Please see customers response below:

I am also looking for recombinant protein to use as positive control in western blot for eNOS/NOS3, eNOS/NOS3 phosphorylated on serine 1177, DDAH1 and DDAH2.

The western blot will be done in de...

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Thank you for your response.

I have conducted a search for your customer and found the following products in our catalogue.

ab109090: eNOS/NOS3 - C terminal peptide, though it has not been tested in Western blot application; it was ...

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Dear Supplier,

We have a customer who is interested ab99863, DDAH1 protein. Please see their inquiry below:

Recombinant protein with a GST-tag/his-tag, are they good positive control ?

The protein is longer with the tag...

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Thank you for your enquiry and interest in our products.

It depends on some factors such as the position of the tag, the number of copies, the clonality of the antibody etc. It would be also interesting to know in what application the custome...

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We have a customer who is looking for recombinant protein to use as positive control in western blot for eNOS/NOS3, eNOS/NOS3 phosphorylated on serine 1177, DDAH1 and DDAH2. Do you have this product?

Thank you for contacting us.

We have:

DDAH1 protein (https://www.abcam.com/ab99863), Recombinant full length Human DDAH1 (amino acids 1-285) with an N terminal His tag.
DDAH2 protein (Tagged-His Tag) (https://www.abcam.com/ab92017...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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