Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Decorin protein
See all Decorin proteins and peptides
Coating ab169898 at 5-10 µg / well (6 well plate) in a specific culture medium may be used for studying Human cells activation / differentiation in vitro.
Purity> 90 % SDS-PAGE.
ab169898 was expressed in E.coli as inclusion bodies, then refolded using “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQMGRGHHHHHHGNLYFQGGEFEASGIGPEVPDDRDFEPSLGP VCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFK NLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTL QELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKL SYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGL SFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNN NISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSA IQLGNYK
Predicted molecular weight39 kDa including tags
Amino acids31 to 359
TagsHis-T7 tag N-Terminus
Our Abpromise guarantee covers the use of ab169898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and glycerol.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Bone proteoglycan II
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab169898 has been referenced in 1 publication.
- Perdu S et al. Maternal obesity drives functional alterations in uterine NK cells. JCI Insight 1:e85560 (2016). PubMed: 27699222